Tuesday, September 02, 2014
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 Nanobiomaterial




Head of department

Dr. Hossein Nazemiyeh, (PharmD; PhD)

About Our Lab

Natural products have been the main members of cancer chemotherapy for the at least last decades. This laboratory is concerned about all experimental works associated to the broadcast and elucidation of novel native compounds extracted from nature and spotlights on the medicinal plants with antioxidant, antimicrobial and anti-cancer properties. Another goal of the Nanobiomaterial lab is assessment of pharmaceutical properties of pure extracted compounds through different cellular and molecular pathways.

The laboratory is also fill the gaps between traditional medicine and high-tech sciences through formulation of natural pure products in different pharmaceutical tagged vehicles such as Solid Lipid Nanoparticles, Magnetic Nanoparticle, Nanostructured Lipid Carrier and so on for targeted drug delivery.  

In this lab, the biotechnological works, which improve the quality and quantity of biomaterials, is also set up.

 

Technical approach

ü  Extraction of natural products

·         Natural product extraction using dry and wet methods

·         Essential oil extraction using Clevenger apparatus

·         Evaporator rotary

ü  Purification of natural products

·         Analytical and preparative- Thin Layer Chromatography (TLC)

·         Vacuum Liquid Chromatography (VLC)

·         Column Chromatography (CC)

·         Semi-Preparative High Pressure Liquid Chromatography (Semi-Prep HPLC) 

ü  Identification of natural products

·         UV-Visible Spectrometry

·         H and C NMR (In collaboration with other research centers)

ü  Improve the quality and quantity of biomaterials

·         Shaker Incubator

·         Class I and II box hoods

ü  Lipidic Nanoparticle synthesizing

·         Ultra-homogenizer

·         Dialysis 

ü  Assessment of natural products properties

·         Anti-oxidant activity assay (DPPH)

·         Anti-microbial activity assays (In collaboration with Molecular Medicine & Therapy Lab)

·         Anti-angiogenesis activity assay (Chorioallantoic Membrane Angiogenesis Assay)


Students

  • Morteza Eskandani, (PhD student), Natural products screening, Nano-drug delivery & genotoxicity, Hypexia inducible factor molecular & cellular targeting
  • Jafar Ezzati Nazhad Dolatabadi, (PhD student), Drug delivery using solid lipid nanoparticles (SLNs) & carbon nanotubes (CNTs), Optical & electrochemical biosensors & nanobiosensors, Cytotoxicity & genotoxicity studies
  • Khadijeh Zare, (PhD student), Plant cell & Tissue culture
 
Recent Publication

2013

Keyhanmanesh, R.Bagban, H.Nazemiyeh, H.Bavil, F.M.Alipour, M.R.Ahmady, M., The relaxant effects of different methanolic fractions of Nigella sativa on guinea pig tracheal chains, Iranian Journal of Basic Medical Sciences, 2013, 16; 123-128.

Hasanzadeh, M., Shadjou, N., Omidinia, E., Eskandani, M., de la Guardia, M., Mesoporous silica materials for use in electrochemical immunosensing, TrAC - Trends in Analytical Chemistry, 2013, 45; 93-106.

 Saei, A.A., Dolatabadi, J.E.N., Najafi-Marandi, P., Abhari, A., de la Guardia, M., Electrochemical biosensors for glucose based on metal nanoparticles, TrAC - Trends in Analytical Chemistry, 2013, 42; 216-227.

Vandghanooni, S., Forouharmehr, A., Eskandani, M., Barzegari, A., Kafil, V., Kashanian, S., Ezzati Nazhad Dolatabadi, J., Cytotoxicity and DNA fragmentation properties of butylated hydroxyanisole, DNA and Cell Biology, 2013, 32; 98-103.


2012

Khodaie, L.Delazar, A.LotfiPour, F.Nazemiyeh, H.Asnaashari, S.Moghadam, S.B.Nahar, L.Sarker, S.D., Phytochemistry and bioactivity of Pedicularis sibthorpii growing in Iran, Brazilian Journal of Pharmacognosy, 2012, 22; 1268-1275.

Naghiloo, S.Movafeghi, A.Delazar, A.Nazemiyeh, H.Asnaashari, S.Dadpour, M.R., Ontogenetic variation of total phenolics and antioxidant activity in roots, leaves and flowers of Astragalus compactus Lam. (Fabaceae), BioImpacts, 2012, 2; 105-109.

Khodaie, L.Bamdad, S.Delazar, A.Nazemiyeh, H., Antioxidant, total phenol and flavonoid contents of two Pedicularis L. Species from Eastern Azerbaijan, Iran, BioImpacts, 2012, 2; 47-53.

Hamedeyazdan, S.Fathiazad, F.Sharifi, S.Nazemiyeh, H., Antiproliferative activity of marrubium persicum extract in the MCF-7 human breast cancer cell line, Asian Pacific Journal of Cancer Prevention, 2012, 13; 5843-5848.

Khodaei, L.Delazar, A.Nazemiyeh, H.Asnaashari, S.Nahar, L.Sarker, S.D., Composition of the volatile oils of the aerial parts of Pedicularis sibthorpii and P. wilhelmsiana growing in Iran, Journal of Essential Oil-Bearing Plants, 2012, 15; 352-356.

Naghiloo, S.Movafeghi, A.Delazar, A.Nazemiyeh, H.Asnaashari, S.Dadpour, M.R., Ontogenetic variation of volatiles and antioxidant activity in leaves of astragalus compactus lam. (fabaceae), EXCLI Journal, 2012, 11; 436-443.

Hassanpouraghdam, M.B.Nazemiyeh, H.Shalamzari, M.S.Mehrabani, L.V., Salt effects on the quality and recovery of Mentha pulegium L. essential oil, Chemija, 2012, 23; 113-117. 

Namazi, N.Esfanjani, A.T.Heshmati, J.Bahrami, A.Nazemiyeh, H., A systematic review about effects of aerial portions of Urtica dioica (nettle) on some cardiovascular risk factors in diabetes mellitus, International Journal of Pharmacology, 2012, 8; 306-313.

Pasdaran, A.Delazar, A.Nazemiyeh, H.Nahar, L.Sarker, S.D., Chemical Composition, and Antibacterial (Against Staphylococcus aureus) and Free-Radical-Scavenging Activities of the Essential Oil of Scrophularia amplexicaulis Benth, Records of Natural Products, 2012, 6; 350-355.

Khodaie, L.Delazar, A.Lotfipour, F.Nazemiyeh, H., Antioxidant and antimicrobial activity of pedicularis sibthorpii boiss. And pedicularis wilhelmsiana fisch ex, Advanced Pharmaceutical Bulletin, 2012, 2; 89-92.

Hasanzadeh, M., Shadjou, N., de la Guardia, M., Eskandani, M., Sheikhzadeh, P., Mesoporous silica-based materials for use in biosensors, TrAC - Trends in Analytical Chemistry, 2012, 33; 117-129.

Hasanzadeh, M., Shadjou, N., Eskandani, M., Guardia, M., Mesoporous silica-based materials for use in electrochemical enzyme nanobiosensors, TrAC - Trends in Analytical Chemistry, 2012, 40; 106-118.

Hasanzadeh, M., Shadjou, N., Eskandani, M., Guardia, M., Room-temperature ionic liquid-based electrochemical nanobiosensors, TrAC - Trends in Analytical Chemistry, 2012, 41; 58-74.

Jamali, A.A., Tavakoli, A., Nazhad Dolatabadi, J.E., Analytical overview of DNA interaction with Morin and its metal complexes, European Food Research and Technology, 2012, 235; 367-373.

Hasanzadeh, M., Shadjou, N., Saghatforoush, L., Dolaabadi, J.E.N., Preparation of a new electrochemical sensor based on iron (III) complexes modified carbon paste electrode for simultaneous determination of mefenamic acid and indomethacin, Colloids and Surfaces B: Biointerfaces, 2012, 92; 91-97.

 

 

 

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